INTENDED USE |
IVD |
SPECIES REACTIVITY |
Human |
SOURCE |
Mouse Monoclonal |
CLONE |
10D6 |
ISOTYPE |
IgG1 |
ANTIGEN |
CD163 |
LOCALIZATION |
Membrane |
POSITIVE CONTROL |
Tonsil or Placenta |
CD163 antibody aids in identifying cells of monocyte/macrophage lineage in
normal and neoplastic conditions.
This antibody reacts with human scavenger receptor cysteine-rich protein
CD163 (p155, M130) found on mononuclear phagocytes including human monocytes and
macrophages. Compared with the CD68 antibodies, studies have shown that CD163
demonstrated greater specificity as a marker of disorders of monocyte/macrophage
origin.
However, immunohistochemical evaluation of CD163 expression does not seem
to be a sensitive means of determining monocytic differentiation of AMLs or
myeloid sarcoma.
Antigen detection, in tissues and cells, is a multi-step
immunohistochemical process.
The initial step binds the primary antibody to its specific epitope. After
labeling the antigen with a primary antibody, an affinity-purified, secondary
antibody is added to bind to the primary antibody.
An enzyme label is then added to bind to the secondary antibody; this
detection of the bound antibody is evidenced by a colorimetric reaction.