MACH 2 Detection is a
one-step / one-solution, biotin-free detection system which combines superior
work flow and sensitivity for mouse and rabbit primary antibodies.
MACH 2 detection
consists of a single reagent applied after the primary antibody. MACH 2 is ideal
for use with prediluted antibodies or concentrates with equal success. MACH 2
may be used for 7-step multiplex stains with certain antibodies.
MACH 2 increases
staining specificity compared to conventional dextran backbone
polymer.
MACH2 Universal can
detect both mouse and rabbit primaries.
The conjugated goat anti-mouse and anti-rabbit
polymer-horseradish peroxidase secondary antibody reacts with heavy and light
chains on mouse and rabbit IgG. It reacts with all mouse and rabbit IgG
subclasses and with mouse and rabbit IgM. The new and innovative
HRP-polymerization technology provides a significant increase in staining
sensitivity when compared to other conventional HRP-conjugated secondary
antibodies. Avidin-biotin blocking procedures are not necessary when using the
MACH 2 conjugated secondary antibodies. The overall staining procedure uses one
less reagent (streptavidin peroxidase) and rinsing step, eliminates
avidin-biotin blocking procedures, and may reduce overall staining time. It can
be used manually and on automated stainers.