The Ferangi Blue Chromogen Kit 2 is provided as a stable, two-component system, consisting of Ferangi Blue Chromogen and Ferangi Blue Buffer. When in the presence of alkaline phosphatase (AP) enzyme, Ferangi Blue Chromogen Kit 2 produces a permanent, bright royal blue precipitate. Typically, Ferangi Blue achieves robust staining intensity in 5-7 minutes. If more intense staining is desired, Ferangi Blue can be extended to 10 minutes, offering users increased flexibility and sensitivity. Ferangi Blue is particularly well-suited for automated multiplex staining applications. Ferangi Blue is partially soluble in xylenes. Slides should be air dried and coverslipped in a xylene-substitute permanent mounting medium, such as Biocare’s EcoMount (EM897).
Ferangi Blue can be coverslipped with a non xylene- or toluene-based permanent mounting media. This chromogen produces a bright royal blue precipitate in the presence of alkaline phosphatase enzyme. For optimum results, use Immunocare TBS Wash Buffer. Not for use with PBS.