L26 (CD20) reacts with a 30-33 kDa
polypeptide present in B-cells. L26 reacts with the majority of B-cells present
in peripheral blood and lymphoid tissues. In normal lymphoid tissue, L26 marks
B-cells in germinal centers, particularly immunoblasts. This antibody has been
shown to be a reliable pan B-cell marker. It rarely marks Tcells.
This rabbit monoclonal CD3 reacts with
the intracytoplasmic portion of the CD3 antigen expressed by T-cells. It stains
human T-cells in both the cortex and medulla of the thymus and in peripheral
L26 and CD3 have been combined to be
used with Biocare’s MACH 2 Double Stain 1. L26 is stained with Fast Red and CD3
is stained with DAB.