p63 encodeD for different isotypes able
to either transactivate p53 reporter genes (TAp63) or act as
p53-dominant-negatives. P63 is detected in prostate basal epithelial nuclei in
normal prostate, however, is negative in malignant tumors of the prostate gland.
Thus p63 is useful as a differential marker for benign and malignant tumors of
prostate gland and can be useful as a negative marker.
The combination of P504S and p63 (PIN)
may be extremely useful for diagnosing prostatic intraepithelial neoplasia
(PIN), especially in difficult and limited tissues cases. This antibody may
eliminate the need for HMW CK [34ßE12]. P504S stains cytoplasm in prostate
adenocarcinoma and atypical adenomatous hyperplasia; p63 stains nuclei in normal
(negative marker) and benign prostate glands. In this instance, the PIN
combination becomes a double stain procedure. (A goat antimouse and rabbit
universal detection kit must be used.)