The
Rat Detection Kit for Anti-Mouse CD31 has been developed for detection of a rat
anti-mouse CD31 primary antibody on mouse tissues.
This kit is composed of blockers, digestion enzyme,
primary antibody diluent, HRP-Polymer, DAB chromogen and hematoxylin.
All reagents supplied with kit are suitable for both
manual and automated techniques. The CD31 primary antibody is available
separately.
Biocare’s Rat-on-Mouse HRP-Polymer Detection is
specially designed for using rat monoclonal antibodies on mouse tissues.
The kit utilizes a biotin-free horseradish peroxidase
(HRP) polymer technology that provides a significant increase in staining
sensitivity. Rat primary antibodies are advantageous on mouse tissues because
mouse adsorbed anti-rat detection systems display minimum cross-reactivity to
endogenous mouse IgG.
The Rat HRP-Polymer Detection is comprised of two
reagents: a Rat Probe that binds to the rat primary antibody; and an HRP-Polymer
that conjugates to the probe. Biocare has developed Rodent Block M for mouse
tissues.
It is a specially formulated blocking reagent that
reduces nonspecific background staining and simultaneously blocks for endogenous
mouse IgG. Rodent Block M is applied prior to the primary antibody for 30
minutes.