Vina Green is a novel permanent chromogen that produces a green stain in the presence of horseradish peroxidase (HRP). The kit consists of liquid Vina Green chromogen and buffer that are stable at least 4 hours at room temperature. Vina Green is clearly distinguishable from Warp Red and DAB on a single slide, enabling high flexibility for its application in Multiplex IHC. Developed for both manual and automated platforms, Vina Green is suitable for both immunohistochemistry (IHC) and in situ hybridization (ISH) applications including HPV, CMV, EBV, p63, TTF-1, Ki-67 and other targets antigens such as blood and lymphatic vessels, and basal and myoepithelial cells.