Rabbit-on-Rodent AP-Polymer has been developed for
use with rabbit monoclonal/polyclonal antibodies on mouse and rat
tissues.
Rabbit primary antibodies on mouse and rat tissues are
advantageous because rabbit secondary detection systems can be used with minimum
cross-reactivity to endogenous mouse and rat IgG.
Advantages of Biocare Medical’s polymer technology are
increased sensitivity, reduction of IHC steps and elimination of endogenous
biotin.
It can be used with formalin-fixed paraffin-embedded
tissues, floating sections, frozen sections or cell cultures. Biocare’s
Rabbit-on-Rodent AP-Polymer is ideal for single staining and may also be used in
double and triple staining techniques. In some tissues such as spleen,
endogenous mouse or rat IgG may be difficult to eliminate.
Biocare Medical has developed an antigen retrieval
solution (Rodent Decloaker) that helps reduce or eliminate endogenous IgG and
non-specific background staining while simultaneously, performing antigen
retrieval. Temperature dependent protocols at 80ºC, 95ºC and 125ºC can be
performed using Biocare’s Decloaking Chamber.
Biocare Medical has additional products to help
eliminate persistent rat and mouse IgG.
The XM and XR Factors are two potent blockers for
eliminating endogenous mouse or rat IgG. By adding 1-2 drops (1 drop equals
33µl) of the XM or XR Factor to 2.5 ml of Rabbit-on-Rodent AP-Polymer detection,
endogenous mouse or rat IgG will be reduced dramatically or completely
eliminated. In addition Rodent Block M (mouse tissue) and Rodent Block R (rat
tissue) block for non-specific background staining and reduce staining due to
endogenous mouse or rat IgG.
Rodent Block M or R is applied to the tissue prior to
the primary antibody for 15 to 30
minutes.