c-erB-2/neu (CB11) 1,0mL

Specificity and Comments:

This antibody recognizes a protein of 185kDa, identified as the second member (c-erbB-2/HER-2) of the c-erbB family. The CB11 monoclonal antibody is directed against the cytoplasmic domain of the human c-erbB-2 protein. The c-erbB-2 is closely related in structure to the epidermal growth factor receptor. The c-erbB-2 protein is over-expressed in a variety of carcinomas, especially those of breast and ovary. Immunohistochemical staining correlates with gene amplification. Studies have shown that c-erbB-2 positive breast cancer usually correlates with negative staining for estrogen and progesterone receptors; thus a poorer predictive outcome is correlated with c-erbB-2 staining.

 

Species Reactivity: Human; others not tested.

 

Clone: CB11

 

Isotypes: IgG1

 

Immunogen:

Synthetic peptide corresponding to a site on the internal domain of the c-erbB-2 oncoprotein

 

Known Applications:

Immunohistochemistry

(formalin–fixed paraffin-embedded tissues)

 

 

 

Positive Control:

Breast carcinoma (Paget’s disease) or a T47D cell line. About 20% to 30% of breast carcinomas are positive for c-erbB-2/HER-2/neu oncoprotein; especially those that are lacking estrogen and/or progesterone receptor.

 

Cellular Localization:

Cell membrane and cytoplasm

 

Supplied As:

Buffer with protein carrier and preservative

 

Storage and Stability:

Store vial at 4°C. Product is stable for 18 months.