Mouse primary antibodies on rat tissues are
advantageous because mouse secondary detection systems can be used with minimum
cross-reactivity to endogenous rat IgG.
Biocare Medical has developed a Mouse-on-Rat alkaline phosphatase (AP)
polymer detection system with minimal cross-reactivity to rat tissues.
It can be used with formalin-fixed paraffin-embedded tissues, floating
sections, frozen sections and cell culture preparations. Biocare Medical has
novel products to help eliminate persistent rat IgG.
XR Factor is a very potent blocker for eliminating endogenous rat IgG. By
adding 1-2 drops (1 drop equals 33 µl) of the XR Factor to 2.5 ml of the
Mouse-on-Rat Polymer, endogenous rat IgG will be dramatically reduced and/or
completely eliminated.
Available as a bundle containing detection polymer, blocking and HIER
solution or available individually.
Supplied as: AP Micro-polymer.