Fast Red is a widely used chromogen for immunohistochemical
staining.
When in the presence of alkaline
phosphatase (AP) enzyme, intelliPATH FLX™ Fast Red produces
a bright
fuchsin-red precipitate that is insoluble in organic solvents---and can be
coverslipped with a permanent mounting media. This product is available in a
stable
two-component system, consisting of Fast Red Chromogen and
Buffer.
When using an alkaline phosphatase system, tris buffer (pH 7.6)
should be used as a
rinsing buffer. PBS should never be used!
Phosphates act as a competitive inhibitor to
alkaline phosphatase enzymes.
For optimum performance, use Immunocare TBS Wash
Buffer with AP activator or TBS Wash Buffer,
20X