Goat-on-Rodent Horseradish Peroxidase (HRP) polymer has been
developed for use with goat primary antibodies on mouse, rat and human tissues.
Goat primary antibodies are advantageous because goat secondary detections
display minimum cross-reactivity to endogenous mouse, rat and human IgG.
Advantages of Biocare Medical’s biotin-free polymer technology are increased
sensitivity, a reduction of IHC steps and elimination of endogenous biotin. It
can be used with formalin-fixed paraffin-embedded tissues, floating sections,
frozen sections or cell cultures. The Goat-on-Rodent HRP-Polymer is comprised of
two reagents: the goat probe that labels the goat primary antibody and an
HRP-polymer that conjugates to the probe. This two-step system has been proven
to be 10-20 times more sensitive than conventional mouse anti-goat HRP secondary
detection systems. Biocare Medical has developed Rodent Block M (for mouse
tissues) or Rodent Block R (for rat tissues) blocking reagents that reduce
nonspecific background staining and endogenous mouse or rat IgG. Rodent Block is
applied to the tissue prior to the primary antibody for 15-30 minutes. For human
tissues, use Background Punisher. Biocare Medical has also developed Rodent
Decloaker, an antigen retrieval solution, which helps reduce and eliminate
endogenous mouse and rat IgG and non-specific background staining while
simultaneously performing antigen retrieval. For human tissues, please refer to
the respective primary antibody data sheet for recommended solutions.
Temperature dependent protocols can be performed using Biocare’s Decloaking
Chamber.