In normal epithelia, HMW Cytokeratins
(CK5 and CK14) stain basal epithelia, myoepithelial cells and basal cells in the
prostate gland and bronchi. p63 is detected in prostate basal epithelial nuclei
in normal prostate, however, is negative in malignant tumors of the prostate
gland. Thus p63 is useful as a differential marker for benign and malignant
tumors of prostate gland and can be useful as a negative marker.
Expression of P504S protein is found in
prostatic adenocarcinoma, but not in benign prostatic tissue. It has also been
found to stain premalignant lesions of the prostate: high-grade prostatic
intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. P504S can
be used as a positive marker for PIN. It will be useful to confirm the diagnosis
of small focus of prostate carcinoma in needle biopsies.
The
combination of HMW CKs + p63 + P504S (PIN-4™) may be extremely useful for
diagnosing prostatic intraepithelial neoplasia, especially in difficult
cases,
and in cases
with limited tissues. P504S stains cytoplasm in prostate adenocarcinoma and
atypical adenomatous hyperplasia, and p63 and HMW CKs stain normal (negative
marker) and benign prostate glands.
*A two-component version (IVD, ASR) can
also be purchased: Prostate-2X Cat. No. PM364 and P504S-2X Cat. No.
PP365