Pan Melanoma Cocktail (HMB45, Tyrosinase and MART-1) 1,0 mL
Specificity and Comments:
The HMB45 clone reacts with a neuraminidase-sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. The HMB45-reactive antigen is present in cutaneous melanocytes, prenatal and infantile retinal pigment epithelium and melanoma cells and is thought to be oncofetal in nature. This antibody has been shown to label the majority of melanomas. The MART-1/Melan A recognizes a protein of 18kDa, identified at MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. Melan-A is a useful addition to melanoma panels as it is apparently specific for melanocytic lesions. Studies have also shown that MART-1 is more sensitive than HMB45 when labeling metastatic melanomas. Tyrosinase is a key enzyme involved in the initial stages of melanin biosynthesis. Studies have shown Tyrosinase to be a more sensitive marker when compared to HMB45 and MART-1. It has also shown to label a higher percentage of desmoplastic melanomas than HMB45. The combination of HMB45 and the MART-1 (cocktail) and Tyrosinase make this quadruple antibody cocktail a first-order pan melanoma screener, and may prove to be a valuable marker for melanoma metastasis in sentinel lymph nodes.
Species Reactivity: Human; others not tested.
Clone: HMB45 + M2-7C10 + M2-9E3 + T311
Isotypes: IgG1/?, IgG2b and IgG2b and IgG2a
Immunogen:
• HMB-45 - Pigmented melanoma metastases from LN
• MART-1 - Recombinant hMart-1 protein
• Recombinant tyrosinase protein
Known Applications: Immunohistochemistry
(formalin-fixed paraffin-embedded tissues)
Positive Control: Metastatic melanoma in lymph node
Cellular Localization: Cytoplasmic
Supplied As: Buffer with protein carrier and preservative
Storage and Stability: Store vial at 4°C. Stable for 18 months