MLH-1 1,0 mL

Specificity and Comments:

The G168-15 antibody recognizes the human and mouse MLH1 (80-85kDa. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. The importance of mismatch repair genes became apparent with the identification of the genetic basis for hereditary nonpolyposis colon cancer (HNPC). MSH-2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair.

Species Reactivity: Human and rat

Clone: G168-15

Isotype: IgG1/kappa

Immunogen:

Full-length recombinant human MLH

Known Applications: Immunohistochemistry (formalin–fixed, paraffin embedded tissues)

Positive Control: Tonsil or colon cancer

Cellular Localization: Nuclear

Supplied As:

1. Buffer with protein carrier and preservative

2. Van Gogh Diluent: 100 ml (Cat# PD902L)

Storage and Stability:

Store vial at 4°C. Stable for 18 months.