Estrogen Receptor (1D5) 1,0ml
Specificity and Comments:
The estrogen receptor (ER) is a 66-kDa protein that mediates the actions of estrogens in estrogen-responsive tissues. It is a member of a large superfamily of nuclear-hormone receptors that function as ligand-activated transcription factors. The ER gene consists of more than 140kb of genomic DNA divided into 8 exons. These translate into a protein with six functionally discrete domains, labeled A through F. A second form of the estrogen receptor, ER? has recently been described. ER [clone 1D5] reacts with the amino-terminal domain in the A/B region of ER?. This clone has been established to work in formalin-fixed, paraffin-embedded tissues and has been published in numerous breast cancer research studies. Staining patterns observed include: ER+ and PR+, ER+ and PR-, ER- and PR+, and ER- and PR-.
(Key Reference: Pertschuk)
Species Reactivity:
Human and rat; others not tested.
Clone: 1D5
Isotype: IgG1/?
Immunogen:
Human recombinant estrogen-receptor protein
Known Applications: Immunohistochemistry (frozens/formalin–fixed paraffin-\ embedded tissues).
FDA Ruling: Class 1
Positive Control: Breast carcinoma
Cellular Localization: Nuclear
Supplied As:
Buffer with protein carrier and preservative
Storage and Stability:
Store vial at 4°C. Stable for 18 months.