| INTENDED USE |
IVD |
| SPECIES REACTIVITY |
Human |
| SOURCE |
Mouse Monoclonal |
| CLONE |
10D6 |
| ISOTYPE |
IgG1 |
| ANTIGEN |
CD163 |
| LOCALIZATION |
Membrane |
| POSITIVE CONTROL |
Tonsil or Placenta |
CD163 antibody aids in identifying cells of monocyte/macrophage
lineage in normal and neoplastic conditions.
This antibody reacts with human scavenger receptor cysteine-rich
protein CD163 (p155, M130) found on mononuclear phagocytes including human
monocytes and macrophages. Compared with the CD68 antibodies, studies have shown
that CD163 demonstrated greater specificity as a marker of disorders of
monocyte/macrophage origin.
However, immunohistochemical evaluation of CD163 expression does not
seem to be a sensitive means of determining monocytic differentiation of AMLs or
myeloid sarcoma.
Antigen detection, in tissues and cells, is a multi-step
immunohistochemical process.
The initial step binds the primary antibody to its specific epitope.
After labeling the antigen with a primary antibody, an affinity-purified,
secondary antibody is added to bind to the primary antibody.
An enzyme label is then added to bind to the secondary antibody; this
detection of the bound antibody is evidenced by a colorimetric
reaction.