Cytokeratin LMW (8/18)(5D3) 1,0mL
Specificity and Comments:
Clone 5D3 recognizes cytokeratin (CK) 8 and 18 intermediate filament proteins. These are 52.5 kD and 45kD respectively. In normal tissues, 5D3 recognizes all simple and glandular epithelium. In neoplastic tissues, 5D3 may prove useful for the identification of adenocarcinomas and some squamous cell carcinomas. Use in conjunction with HMW CK (Cat. #PM026AA-clone: DESQ) to rule out squamous cell carcinoma. Keratinizing squamous carcinomas are generally negative. It works well in formalin-fixed, paraffin-embedded tissue sections.
Species Reactivity: Human; others not tested.
Clone: 5D3
Isotype: IgG1
Immunogen: Cytokeratins from the human breast carcinoma cell line MCF-7.
Hybridoma:
Produced by fusion between BALB/c splenocytes and mouse myeloma p3-NS1-Ag4-1
Known Applications: Immunohistochemistry (formalin-fixed paraffin-embedded tissues)
Positive Control: Skin
Cellular Localization: Cytoplasmic
Supplied As:
Buffer with protein carrier and preservative
Storage and Stability:
Store vial at 4°C. Stable for 18 months.