Denaturing Solution is used as an elution step when
using a double stain technique
(Biocare’s 7-step double stain procedure).
This will ensure that the second staining protocol will not cross react
with the first staining protocol.
Solution A (yellow) is a concentrate that must be diluted with Solution B
(red, dilution buffer) for proper elution.
It is recommended that the Denaturing Solution be used at different
dilutions depending on tissue fixation and the type of antigen retrieval method
used.